Graphs representing main and significant results from Table 1. I. Activation in culture by follicular counts. a Results are in percent of primordial follicles. Primordial follicle count is significantly higher in thawed-untreated samples than after culture with basic medium alone, regardless of the matrix (P < 0.05). b Results are in percent of developing follicles. Developing follicle count is higher in samples cultured with basic medium alone than in thawed-untreated samples, regardless of the matrix (NS). II. PEG-fibrinogen hydrogels versus alginate scaffolds. c Results are in percent of atretic follicles. Atretic follicle count is significantly higher on alginate scaffolds than atretic follicles in PEG-fibrinogen hydrogels, regardless of treatment (P < 0.04). d Results are in percents of developing follicles. Developing follicle count is higher in samples cultured in PEG-fibrinogen hydrogels than in thawed-untreated samples (NS). e Results are in percent of Ki67 positively stained follicles. The proportion of stained follicles is significantly higher on alginate scaffolds or in PEG-fibrinogen hydrogels with basic medium than in thawed-uncultured samples (P < 0.0001). III. Destructive effects of bpV (pic). Results are in percent of primordial follicles. f Primordial follicle count is significantly higher in thawed-untreated samples than in samples cultured with bpV (pic), regardless of the matrix (P < 0.05). (G) Developing follicles count is significantly higher in samples cultured in PEG-fibrinogen hydrogel with 740Y-P than in PEG-fibrinogen hydrogel with bpV (pic) (P < 0.05). h E2 level is significantly higher in samples cultured with 740Y-P (P < 0.05) or with basic medium alone (P < 0.03) than with bpV (pic), regardless of the matrix. IV. Thick versus thin samples. Results are in pg/ml. Values represent mean±standard deviation. i E2 level is significantly higher after culture of thick slices than thin slices (P < 0.0004), regardless of the matrix. j E2 level is significantly higher after culture of thick slices than thin slices on alginate scaffolds (P < 0.04)