Fig. 3.

Histological and Ki67 immunostaining of uncultured and cultured ovarian samples. a Thawed-uncultured ovarian section from a 22-year-old woman. Note the primordial follicles. Hematoxylin and Eosin staining, original magnification X400. b Section of ovarian sample with Ki67 immunohistochemical staining from a 13-year-old girl cultured in PEG-fibrinogen hydrogel for 1 week. Note the small secondary follicle, with the light red-brown staining indicating Ki67 expression in a portion of the granulosa cells (arrow). Original magnification X400. c Section of ovarian sample with Ki67 immunohistochemical staining from the same patient as in panel (A) cultured in PEG-fibrinogen hydrogel for 1 week. Note the primary and primordial follicles, with the red-brown staining indicating Ki67 expression in a portion of the granulosa cells (arrows). Original magnification X400. d Section of ovarian sample from a 10-year-old girl cultured on alginate scaffold with 740Y-P for 1 week. Note the two primordial-primary follicles (arrow) and the three atretic follicles (arrow heads). Hematoxylin and Eosin staining, original magnification X400. e Section of ovarian sample from the same patient as in panel (B) cultured on alginate scaffold for 1 week with bpV (pic). Note the atretic follicles (arrows). Hematoxylin and Eosin staining, original magnification X400