Table 2.
Follicular counts in samples and E2 secretion after 1 week
| Study condition | Treatment | Primordial follicles | Developing follicles | Atretic follicles | Total | E2 (pg/ml) (mean±SD) |
|---|---|---|---|---|---|---|
| Thawed | – | 161a (74 %)* | 48b (22 %) | 8 (4 %) | 217 | – |
| 12 ± 3** | 4 ± 1 | 1 ± 0.2 | 17 ± 5 | |||
| Matrix | ||||||
| Basic medium | 94 (51 %) | 55 (30 %) | 35 (19 %) | 184 | 251 ± 89c | |
| 5 ± 1 | 3 ± 1 | 2 ± 0.4 | 9 ± 2 | |||
| Alginate Scaffoldd | +bpV (pic) | 0 | 3 (15 %) | 17e (85 %) | 20 | 0.5 ± 0.4 |
| 1 ± 0.3 | 3 ± 2 | 4 ± 2 | ||||
| +740Y-P | 147 (38 %) | 218 (57 %) | 19 (5 %) | 384 | 318 ± 134c | |
| 12 ± 4 | 18 ± 5 | 2 ± 0.5 | 32 ± 9 | |||
| Basic medium | 92 (33 %) | 197 (68 %) | 2 (1 %) | 291 | 174 ± 70c | |
| 7 ± 2 | 14 ± 4 | 0.14 ± 0.04 | 21 ± 6 | |||
| PEG-Fibrinogen | +bpV (pic) | 1 (33 %) | 0 | 2 (67 %) | 3 | 36 ± 35 |
| 0.3 ± 0.2 | 1 ± 0.4 | 1 ± 1 | ||||
| +740Y-P | 83 (55 %) | 56f (37 %) | 11g (7 %) | 150 | 149 ± 38c | |
| 14 ± 6 | 9 ± 4 | 2 ± 1 | 25 ± 10 | |||
*Sum of follicles per treatment group. Percents were calculated from the total number of follicles in the treatment group
**Mean±SD per sample
aSignificantly higher primordial follicle count in thawed-untreated samples than after culture with bpV (pic) or basic medium alone, regardless of the matrix (P < 0.05)
bSignificantly higher developing follicle count in thawed-untreated samples than after culture with bpV (pic), regardless of the matrix (P < 0.04)
cSignificantly higher E2 level after culture with basic medium alone (P < 0.03) or with 740Y-P (P < 0.05) than with bpV (pic), regardless of the matrix
dSignificantly higher atretic follicle count on alginate scaffolds than atretic follicles in PEG-fibrinogen hydrogels, regardless of treatment (P < 0.04)
eSignificantly higher atretic follicle count on alginate scaffolds with bpV (pic) than atretic follicles in thawed samples (P < 0.05) and in PEG-fibrinogen hydrogels, regardless of treatment (P < 0.04)
fSignificantly higher developing follicles count in PEG-fibrinogen with 740Y-P than developing follicles in PEG-fibrinogen hydrogels with bpV (pic) (P < 0.05) and in thawed samples (P < 0.03)
gSignificantly higher atretic follicle count in PEG-fibrinogen with 740Y-P than atretic follicles in PEG-fibrinogen hydrogels with basic medium (P < 0.02)