Fig. 2.

Laminar specificity of MR diffusion histology images. An intensity plot reveals laminar features on a T₂-weighted (T2w) image (blue line) and ADC (red line) map. On the T2w image, a hyperintense line separates the molecular cell layer (I) and granular cell layer (III). This cytoarchitectural feature (blue arrows) represents the Purkinje cell layer (II). However, this layer is not evident on the ADC map, but here the molecular (I), granular cell layer (III, red arrows) and white matter can be clearly separated as distinct regions. These signal intensity changes further corroborate the ability of diffusion MRI to discriminate cytoarchitectural features at high resolution and characterise microstructural properties of each individual cerebellar layer