Fig. 4.

Role of STIM 1 and Orai 1 in store-operated Ca²⁺ entry (SOCE) in primary-cultured glomerular mesangial cells from young rat. a–c Representative traces showing 1 mM Ca²⁺ application-induced [Ca²⁺]_i rise in scrambled siRNA control (a Con-siRNA), Orai 1 (b), and STIM 1 (c) siRNA-transfected GMCs treated by 4 μM thapsigargin for 10 min in Ca²⁺-free saline solution. d–f Summary of data showing changes in [Ca²⁺]_i rise in response to extracellular Ca²⁺ application after the treatment of TG (d), angiotensin II (e), or glucagon (f) in Ca²⁺-free saline solution in young and aged GMCs. Mean ± SE. **P < 0.01 compared to GMCs transfected with scrambled siRNA control, n = 3–9