Fig. 5.

Ca²⁺ store depletion-induced STIM 1 and Orai 1 puncta formation and co-localization. Primary-cultured mesangial cells from young and aged rats were treated by 4 μM thapsigargin, 1 μM angiotensin II, or 10 nM glucagon for 10 min in Ca²⁺-free saline solution and fixed. Cells were stained with anti-STIM 1 and anti-Orai 1 antibody. After subtracting background (omission of primary antibody), fluorescent signals represent STIM 1 (green) and Orai 1 (red) proteins