FIG. 4.

Total hair cell number increased upon DAPT treatment in postnatal and adult cristae. A Maximum intensity projections of Gfi1⁺ hair cells in explants from P7 and P30 mice after 5 DIV with 30 μm DAPT or DMSO. Scale bars 100 μm. Arrows point to regions of increased hair cell density. B At each age examined, the total number of Gfi1⁺ hair cells was significantly increased in DAPT-treated cristae versus DMSO controls (Table 1). Note that the scale on the y-axis begins at 600. Error bars depict SEM. One-tailed unpaired Student's t test where *p ≤ 0.025 and **p ≤ 0.0125. C The difference in hair cell number between treated and control cristae was similar at all ages. Error bars depict SE.