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Binding activity and specificity of mini-FH and FH-derived proteins to C3 opsonins. (A) Relative binding of FH, mini-FH, FH1-4 and FH19-20 to deposited opsonins C3b, iC3b and C3dg as determined by SPR. Responses were normalized to the highest signal within each set of analyte. (B) SPR-derived apparent binding affinities of all four analytes for C3b. Steady state responses were fitted to a single-site model and normalized for binding capacity.
