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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Cancer Res. 2013 Aug 12;73(19):10.1158/0008-5472.CAN-13-0368. doi: 10.1158/0008-5472.CAN-13-0368

Figure 1. Nm23 inhibits the in vitro motility of 4T1 mammary carcinoma cells.

Figure 1

A. Boyden chamber motility assay using parental 4T1 cells, two independent clonal vector transfectants (VC1, VC2), and two independent clonal transfectants each of Nm23-H1 (human homolog, H1a and H1b) and Nm23-M1 (murine homolog, M1a, M1b). Cells migrated to 1% FBS for 4 hours. Nm23-H1 overexpression significantly inhibited motility as compared to vector control transfectants, P= 0.0002 and P < 0.0001, respectively. Data from three experiments are shown. B. Using anti-Flag antibody, proteins binding transfected Nm23-H1-Flag or Nm23-M1-Flag were pulled-down from lysates of transfected 4T1 cells in vitro (left panel) and from mfp primary tumors (right panel), and separated by electrophoresis. After elution, Nm23 binding proteins were identified using mass spectrometry.