Table 1.
Selected Nm23-H1 and –M1 binding proteins in 4T1 murine mammary carcinoma cells in vitro and in vivo1.
| Protein: | Accession #: | # Peptides Sequenced in Nm23-M1/Nm23-H1 Co-immunoprecipitations2: | Related Co-immunoprecipitating Proteins Also Identified: | Validation4: | ||
|---|---|---|---|---|---|---|
| Cell Culture | Primary Tumor | Metastasis3 | ||||
| Ezrin | P26040 | 2/5 | 17/12 | 0/0/0 | Co-IP | |
| Gelsolin | P13020 | 52/75 | 6/0/0 | Co-IP | ||
| Actin related protein (Arp) 3 | Q99JY9 | 3/32 | 11/21 | 1/0/0 | Arp 2/3 complex subunits 1b, 2, 4, 5 | |
| Eukaryotic initiation factor 4A-1 | P60843 | 3/0 | 9/25 | 0/0/0 | EIFs 2C2, 2-1, 3A, 3E. 3F, 3H, 5A1 | Nonspecific |
| Heat shock protein 90α | P07901 | 0/0 | 11/14 | 1/1/0 | (45) (indirectly) | |
| Casein kinase II, subunit α | Q60737 | 2/6 | 0/4 | 0/0/0 | (46) | |
| Tubulin β6 | Q922F4 | 0/0 | 6/2 | 0/0/0 | (47) | |
| Rab GDI α | P50396 | 0/0 | ½ | 0/0/0 | Rab GDIβ | |
| Rho GEF19 | Q8BWA8 | 1/0 | 1/3 | 0/0/0 | (48) | |
| Guanine nucleotide binding G(i), α2 | P08752 | 5/10 | 3/6 | 0/0/0 | Subunit β1 | (49) |
| Ser/Thr protein phosphatase PPI-α | P62137 | 2/16 | 2/7 | 0/0/2 | PPI 12A | |
| Fatty acid synthase | P19096 | 6/36 | 17/45 | 12/0/0 | Nonspecific | |
| AP-2 complex, a2 subunit | P17427 | 3/0 | 5/16 | 0/0/0 | Subunit β1 | |
| Histone H4 | P62806 | 1/0 | 9/23 | 0/1/0 | H1.2, H2A.1F, H2B.1B | |
| Lyn | P25911 | 0/4 | ¼ | 0/0/0 | Nonspecific | |
| Syk | P48025 | 0/0 | 0/2 | 0/0/0 | Nonspecific | |
| ATP-citrate lyase | Q91V92 | 6/12 | 1/5 | 0/0/7 | Other metabolic enzymes: isocitrate dehydrogenase, 6-phosphofructokinase, lactate dehydrogenase, hexokinase, transketolase | |
| Phosphoglycerate mutase | Q8BX10 | 1/5 | ½ | 0/0/0 | (50) | |
1
Co-immunoprecipitating proteins listed when multiple peptides were identified as binding to a Nm23 protein lysate.
2
Number of peptides sequenced from Nm23-M1/Nm23-H1 expressing transfectants, minus peptides sequenced from control vector transfectant.
3
Lymph node/Spleen/Liver metastasis peptides from Nm23-H1 and –M1 combined.
4
Published reports or unpublished co-immunoprecipitation (Co-IP) experiments. Nonspecific indicates a lack of a co-immunoprecipitation in unpublished experiments.