Fig. 7.

Downregulating AmOA1 octopamine receptors abolishes the octopamine effect. a With control dsRNA injection, and in the non-injected control (surgery), the increase in spontaneous activity was more pronounced than when AmOA1 receptor levels were downregulated by dsRNA injection. Data are normalized to pre-OA values (set to 1). Mean and SEM. See “Results” for statistical tests. b Background calcium levels were not affected in a systematic way. Mean and SEM. See “Results” for statistical tests. c, d When glomeruli are grouped as giving negative, weak or strong responses (compare with Fig. 5), odor-induced responses in negative and weak signals were higher for control animals than for Amoa1 dsRNA injected animals, although this effect was not significant due to high variability in odor-response strength (note the error bars). Mean and SEM. See “Results” for statistical tests. e In an independent dataset, OA had a highly significant effect in control animals, which was abolished by Amoa1 dsRNA treatment. Buffer control animals were injected with injection buffer only, and no dsRNA. Mean and SEM. See “Results” for statistical tests