Fig. 3.

Noxa regulates survival of murine erythroid progenitor cells under cytokine limiting conditions. a Relative gene expression of sorted bone marrow derived murine MEPs (Lin⁻CD117⁺CD34⁻CD16⁻) and splenic CD71⁺ cells, compared to the expression in HSC/CMPs (Lin⁻CD117⁺CD34⁺CD16^Dim), measured by RT-MLPA. Data is expressed on a logarithmic scale. Error bars represent SEM (n = 3). b FDM cells generated from WT or Noxa^−/− mice were deprived of IL-3 for 2 days. Viability was determined by staining for sub-G1 DNA containing cells. Data represent the mean of 5 experiments. Error bars represent SEM. c Western blot analysis of Mcl-1 levels in WT- or Noxa^−/−-FDM cells cultured for 2 days in the presence of the minimal IL-3 concentration that sustained full proliferation and survival (+IL-3), 10 % of that concentration (0.1 × IL-3) or in absence of IL-3 (−IL-3). β-Actin is used as a control for equal loading