Fig. 6.

Increased expansion of erythroid progenitors in a model for inflammation-induced stress-erythropoiesis. Noxa^−/− mice were crossed with CD70^TG mice, to generate Noxa70 mice and were compared with WT, Noxa^−/− and CD70^TG animals. a Hematological blood parameters of 12 week old mice. b Splenic weight and c number of white blood cells in the spleens of mice at 8, 12 and 16 weeks of age. d HE staining of spleens of mice at 8 weeks of age (25× enlarged). e Absolute number of the erythroid progenitor populations Pro-E (CD71⁺Ter119^Dim), Ery-A (CD71⁺Ter119⁺FSC^High) and Ery-B (CD71⁺Ter119⁺FSC^Low) in 8 week old mice. f Representative FACS plots of data shown in e. Gated is for nucleated cells (i.e. Ery-C and mature red blood cells are excluded). Right most plot shows which gates contain Erythroblast subsets. 3–4 animals are used per experiment. Error bars represent SEM. * p < 0.05, ** p < 0.005, *** p < 0.0005 (Student’s t test)