Fig. 3. GPF prevents H2O2-induced apoptosis in HaCaT keratinocytes. (A) HaCaT cells were treated with GPF (20 μM) or NAC (2 mM) and exposed to H2O2 (1 mM) 1 h later. After incubation for 24 h, cell viability (expressed as a percentage of total cells) was determined by the MTT assay. (B) Apoptotic bodies (arrows) were observed by fluorescence microscopy and quantified in cells stained with Hoechst 33342. All measurements were performed in three independent experiments. *Significantly different from control cells (p<0.05), #Significantly different from H2O2-treated cells (p<0.05).
