Figure 1.

Dose-dependent response of selected yeast strains to Mn²⁺. Wild-type parental strain (BY4741) was tested for growth on media after exposure to 0, 0.5, 1.5, 2.5, and 5 mM Mn²⁺ as indicated in Materials and Methods. Survival was determined by counting the number of colonies in the respective dilutions and calculated based on the growth of cells not exposed to Mn²⁺ (100% survival). Mutant strains ntg1 and hsp104 that display increased and reduced sensitivity to Mn²⁺, respectively, are shown for comparison. The curve was fitted by nonlinear Sigmoidal dose response (variable slope).