Figure 2.

Sensitivity of yeast strains to Mn²⁺. (a) The survival of the strains in 1.5 mM Mn²⁺ was determined as described in Section 2. Serial dilutions (1 : 10–1 : 10⁵) of treated cultures were spotted on YPD plates. Growth was scored after 3 days of incubation at 30°C. The serial dilutions of the strains are shown. (b) Quantification of the survival of the tested strains. Survival was determined by counting the number of colonies in the respective dilutions and calculated based on the growth of strains not treated with Mn²⁺. Strains are presented as being ordered from least to more sensitive. Wild-type strain is depicted by a black bar.