Fig. 6.
Effect of silibinin on cell viability and LPS-stimulated IL-6 expression in RAW264.7 mouse macrophage cells. The RAW264.7 cells (1 × 106 cells/ml) were pretreated with indicated concentrations of silibinin (0.05 to 0.4 mM) for 1 h and then incubated with LPS for 24 h. a Cell viability was determined with an MTS assay. b The IL-6 secreted protein level in the supernatant was measured by the ELISA assay. Each data point presents the mean ± SD of three independent experiments (***p < 0.001 compared to control). N no treatment, C induced control