Fig. 5.

γ-H2AX results obtained for HeLa cells treated with Etoposide in presence and absence of 200nM LTUSI54. a Flow cytometry results following 4 h treatment alone with Etoposide, 200nM LTUSI54 alone and in combination or (b) 4 h treatment followed by 2 days in recovery media. Left hand gate indicated cells that are considered to have been negative for γ-H2AX antibody, while right hand gate indicates cells that were considered to have stained positive for γ-H2AX antibody. c Percent of cells stained positive for γ-H2AX following 4 h treatment alone and (d) 4 h treatment followed by 2 days recovery. Graphs indicate mean of three replicates ± SEM. Statistical significance was determined using two-tailed T tests. (Asterisk) Indicates treatments significantly different to control