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. Author manuscript; available in PMC: 2014 Apr 23.
Published in final edited form as: Nat Commun. 2013;4:2626. doi: 10.1038/ncomms3626

Figure 4. CldU incorporation and Cdkn1b expression in the ileums of doxycycline treated R26-M2rtTA;TRE-Tight-Cdkn1b mice.

Figure 4

M2rtTA;TRE-Tight-Cdkn1b mice were either untreated (panel A), or treated at two months of age with 1 mg/ml doxycycline continuously for 3d (panels B and E), 2 weeks (panels C and F) or 5 months (panel D). Paraffin sections were prepared and stained for CldU incorporation (green) and Cdkn1b (red) in panels A–D and F, or CldU (green) and IdU (red) incorporation in panel E, and images were captured using a 20× objective. In panel G the frequency with which Mcm2+ nuclei (see Supplementary Figure S2) at various positions from the bases of the crypts label with CldU following a 2 hour pulse is quantified for control (blue squares) and Cdkn1b over-expressing (red triangles) mice. In panels H–K the distribution of crypts containing different numbers of nuclei incorporating CldU is plotted for untreated (Panel H) and 3d (Panel I), two week (Panel J) and 5 month (Panel K) doxycycline treated mice.