Fig. 3.

T cells, but not macrophages or epithelial cells, display enhanced IL-6 production following Am80-treatment in vitro. a Wild-type C57BL/6 mice were infected with a high dose of T. muris eggs and MLN cells extracted at day 21 p.i.. 1 × 10⁶ T cells were stimulated using anti-CD3 (3μg/ml) and anti-CD28 (5μl/well) antibodies, and treated with vehicle, Am80 (1μM) or Am80 (100 nM) in vitro for 48 h. Similar data was obtained in an independent experiment sourcing T cells from a low dose infection. b Bone marrow-derived macrophages were grown from wild-type C57BL/6 mice and 0.5 × 10⁶ were stimulated in vitro with T. muris Excretory-Secretory antigens (E/S) with or without Am80 (1 μM) or Am80 (100 nM) for 48 h. c The mouse rectal epithelial cell line CMT93 was grown and 0.5 × 10⁶ cells were stimulated in vitro with T. muris Excretory-Secretory antigens (E/S) with or without Am80 (1 μM) or Am80 (100 nM) for 48 h. Values are means + SEM. The data are representative of two independent experiments. (n = 3–4). *p < 0.05, ***p < 0.001, ns = no significance