Figure 3.

HPTLC analysis of staphyloxanthin in crude methanol extract from Staphylococcus aureus. Crude methanol extracts of staphyloxanthin from the S. aureus white variant (W), the S. aureus white variant co-cultured for 48 hours with Pseudomonas aeruginosa 6611 (Wp), and the S. aureus yellow variant (Y) were analyzed by HPTLC on silica plates (pore size 60 Å). The results indicated a significant difference in pigment-band intensity between the white variant and the white variant co-cultured with P. aeruginosa 6611.

Notes: Numbers below the bands represent the absorbance of the crude methanol extracts at A₄₆₅ (W = 0.121, Wp = 0.463, Y = 0.381). There is ~4-fold increase in absorbance in crude extracts of the white variant after co-culture with P. aeruginosa 6611, indicating substantial increase in pigment production.

Abbreviation: HPTLC, high performance thin layer liquid chromatography.