Table 1.
Sequencing and qRT-PCR primers
| Overlapping PCR primers | Sequence | Reference |
|---|---|---|
| C1 F | GGTCCATGTTCGATTTTACG | This study |
| C1 R | TAAAACAGGTAGCGGAATCG | |
| C2 F | TTGGCGTAAAGATTGTTTCTG | This study |
| C2 R | AGAACGTATCGCAAACCAAG | |
| C3 F | TCAATTAGCTGATCTGCATGATT | This study |
| C3 R | GGATATTGTTATGGTGTTGCTGG | |
| C4 F | AAATCTTCACCGACATCTCTT | This study |
| C4 R | TGCCCGTAACAGTTTATGAAGG | |
| C5 F | ATAGAACCGAACAACCACAACAC | This study |
| C5 R | GCAGACAAAATAAACGCGC | |
| C6 F | AATGCACCACACGCCATAG | This study |
| C6 R | ACTTGCGACAAATGTTAGGC | |
| C7 F | AAGGCATTGGCTAAATGATGG | This study |
| C7 R | ATAGAGACAAAGAGGGCAGAGT | |
| C8 F | AACTGCGTTAATCTCGGTCG | This study |
| C8 R | TGTTGGTGGGAAAATTGGTTC | |
| Universal primers | ||
| 27 F | AGAGTTTGATCMTGGCTCAG | Marchesi et al33 |
| 1392 R | ACGGGCGGTGTGTRC | |
| qRT-PCR primers | ||
| crtM F | CTGCTAATTCTATGATTGGTTGTGC | This study |
| crtM R | TGGGAATATTATGCAGCTATMGCAG | |
| sigB F | GAAATWGGRCCWAGAATMAA | This study |
| sigB R | TTTGTCCCATTTCCATYGCT | |
| sarA F | GTTTTCGCTGATGTATGTCA | This study |
| sarA R | TCAATGGTCACTTATGCTGACA | |
| FPP synth F | GCGGTCAAATGTTAGATATGCAA AGCG | This study |
| FPP synth R | ATCTGCTGCACTCATAACCGCAA | |
| hfq F | CGAAAACATCCAAGACAAAGC | This study |
| hfq R | AAGTGCTGATCGCATGTTTG | |
| katA F | TGCATGCCAAAGGTTCTGGTGC | This study |
| katA R | ACGGGCAAACATTTCGGTTTGCTT | |
| agrA F | TGCGAAGACGATCCAAAACAAA GAGA | This study |
| agrA R | GCTTGCTCAAGCACCTCATAAG GATT | |
Abbreviations: PCR, polymerase chain reaction; qRT-PCR, quantitative reverse transcription polymerase chain reaction; crtM, carotenoid biosynthetic gene M; sigB, sigma B transcription factor gene; sarA, staphylococcal accessory regulator A gene; FPP synth, farnesyl pyrophosphate synthase gene; hfq, host factor gene for Q beta; katA, catalase gene A; agrA, accessory gene regulator A; F, forward; R, reverse.