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. Author manuscript; available in PMC: 2014 Oct 15.
Published in final edited form as: J Immunol. 2013 Sep 16;191(8):10.4049/jimmunol.1300298. doi: 10.4049/jimmunol.1300298

Figure 1. Palmitic acid induces pro-IL-1β expression and inflammasome-mediated IL-1β secretion in THP-1 monocytes.

Figure 1

(A and B) THP-1 cells were serum starved in 0.25% FBS-RPMI-1640 for 12 hours then treated with C16:0 (150 µM) for the indicated times. (A) Cell lysates were immunoblotted for pro-IL-1β, NALP3, and caspase-1. (B) IL-1β in cell culture supernatant was analyzed by ELISA and immunoblot. Cleaved caspase-1 in supernatant was analyzed by immunoblot. (C) Serum starved THP-1 cells were treated with indicated concentrations of C16:0. After 6 hours cell lysates were immunoblotted for pro-IL-1β. After 24 hours cell culture supernatants were analyzed for IL-1β by ELISA. Data in B and C are expressed as mean ± s.d. and are representative of three independent experiments with similar results. (D) Serum starved THP-1 cells were incubated with caspase-1 inhibitor (Ac-YVAD-AOM) for 1 hour then treated with C16:0 for 24 hours. IL-1β in supernatant was analyzed by ELISA. Data are expressed as mean ± SEM of three independent experiments. Significance was determined by ANOVA (#P < 0.001 significantly different from untreated, *P < 0.05, **P < 0.01 significantly different from C16:0).