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. Author manuscript; available in PMC: 2014 Oct 15.
Published in final edited form as: J Immunol. 2013 Sep 16;191(8):10.4049/jimmunol.1300298. doi: 10.4049/jimmunol.1300298

Figure 2. Palmitic acid induces but Docosahexaenoic acid (DHA) inhibits the expression of pro-IL-1β and inflammasome-mediated IL-1β production.

Figure 2

(A and B) THP-1 cells were serum starved in 0.25% FBS-RPMI-1640 for 12 hours. Cells were incubated with DHA for 1 hour then treated with C16:0 (150 µM). (A) After 6 hours cell lysates were immunoblotted for pro-IL-1β and caspase-1. (B) After 24 hours cell culture supernatants were analyzed for IL-1β by immunoblot and ELISA. Secreted caspase-1 was analyzed by immunoblot. Data are expressed as mean ± s.d. and are representative of three independent experiments with similar results.