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. Author manuscript; available in PMC: 2014 Oct 15.
Published in final edited form as: J Immunol. 2013 Sep 16;191(8):10.4049/jimmunol.1300298. doi: 10.4049/jimmunol.1300298

Figure 4. Pam3CSK4 induces pro-IL-1β expression and inflammasome-mediated IL-1β secretion in THP-1 monocytes.

Figure 4

(A) THP-1 cells were serum starved in 0.25% FBS-RPMI-1640 for 12 hours then treated with Pam3CSK4 (10 ng/ml) and C16:0 (150 µM) for the indicated times. Cell lysates were immunoblotted for pro-IL-1β. (B) Serum starved THP-1 cells were treated with Pam3CSK4 at indicated concentrations for 24 hours. Cell culture supernatants were analyzed for IL-1β by ELISA. Data in A and B are expressed as mean ± s.d. and are representative of two independent experiments with similar results. (C and D) Serum starved THP-1 cells were incubated with caspase-1 inhibitor (Ac-YVAD-AOM) (C) or DHA (D) for 1 hour then treated with Pam3CSK4 for 24 hours. Cell culture supernatants were analyzed for IL-1β by ELISA. Data in C and D are expressed as mean ± SEM of three independent experiments. Significance was determined by ANOVA (#P < 0.001 significantly different from untreated, *P < 0.05, **P < 0.01, ***P < 0.001 significantly different from Pam3CSK4).