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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: J Vasc Surg. 2013 May 14;58(6):10.1016/j.jvs.2013.02.241. doi: 10.1016/j.jvs.2013.02.241

Figure 3.

Figure 3

Immunostaining for NETs in murine hind limb IR injury using the monoclonal anti-histone H2A/H2B/DNA complex antibody; representative images are shown. (a) WT tissue sections show extensive immunostaining for NETs, indicated by brown color, in interstitial tissue (arrowhead) and in thrombi (arrow) within vessels. There is increased cellular density in regions of intense NETS detection. (b) TLR4m tissue section showing minimal if any interstitial (arrowhead) and intravascular NETs (arrow) immunostaining. (c) Contralateral hind limb muscle fibers are normal with positive immunostaining only in the nuclei of muscle fibers. (d) Negative control. Black bar in each image represents 200 microns.