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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: J Vasc Surg. 2013 May 14;58(6):10.1016/j.jvs.2013.02.241. doi: 10.1016/j.jvs.2013.02.241

Figure 4.

Figure 4

Immunofluorescent images show NETs in red and nuclear DNA in blue in WT tissue section with and without DNAse treatment. (a) WT tissue section demonstrates detection of NETs in a thrombosed vein (arrowhead) and in the interstitium (arrows). (b) Adjacent tissue section was immunostained for NETs following an initial incubation with DNase enzyme. The section shows significant decrease in NETs signal suggesting that nuclease pretreatment markedly reduced the level of antigen available for detection. The white bar in each image represents 80 microns.