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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: J Vasc Surg. 2013 May 14;58(6):10.1016/j.jvs.2013.02.241. doi: 10.1016/j.jvs.2013.02.241

Figure 6.

Figure 6

(a) Relative detection of p65 NF-κβ activity using an ELISA assay in murine hind limb muscle tissue following IR. TLR4m mice had less p65 NF-κβ activity compared to WT mice following 1.5 hours of ischemia and one hour of reperfusion (*p=0.0025). By 48 hours there was no difference in p65 NF- κβ activity detected between the two groups. (b) IκBα protein levels were quantitated using a Western blot analysis following 1.5 hours of ischemia and 1 hour of reperfusion. TLR4m mice had significantly greater levels of total IκBα in reperfused skeletal muscle compared to WT mice (*p<0.01). In contrast there was no significant difference in the Ser32-phosphorylation of IκBα. (c) Representative Western blot analysis of three WT and three TLR4m derived muscle tissues. (d) TLR4m mice showed significant decrease in the poly ADP ribose-modified proteins at 48 hours reperfusion compared to WT (*p=0.032). (e) Representative Western blot image of the detected poly ADP ribose-modified proteins in three WT and three TLR4m skeletal muscle tissues.