Levels of CD68, Iba-1, and MHC II were increased in the brains of eNOS−/− mice. (A) Brain tissue from 4 month or 15 month old eNOS−/− and wild type mice was Western blotted using anti-CD68, anti-Iba-1, anti-MHC II, or anti-Actin (loading control) antibodies. Representative image is shown. Densitometric analysis was performed for (B) CD68, (C) Iba-1, and (D) MHC II. Data are presented as mean ± SD (n=8 animals, *P<0.05 compared to age-matched wild type control; #P<0.05 based on genotype: 2-way ANOVA, followed by Tukey-Kramer post hoc tests for individual comparisons).