Representative images illustrating different subpopulations of EEC cells in sea
bass gastric and intestinal mucosa. Somatostatin (SOM), substance P (SP),
calcitonin gene-related peptide (CGRP), cholecystokinin (CCK) and
α-transducin (Gαtran) (a–h) labelled cells in
the stomach (a, arrows) and intestinal mucosa (b, c, d, g, arrows and h,
arrowhead), respectively. CGRP and CCK immunolabelled nerve fibers (arrowheads)
were observed in the stomach (e) and in the submucosal layer of the intestine (c
and f). Some labelled CGRP (e) and CCK (f) neuronal cell bodies (arrows) were
identified in the muscular layer. Arrow in g indicates a
Gαtran-IR cell negative for SOM (h) in the intestinal
mucosa and arrowheads in h point to SOM immunolabelled cells. a, b, e, and h
scale bars = 30 μm; c, d scale bars = 60 μm; f
scale bars = 20 μm