Table 1.
List of antibodies used in this study.
| Primary antisera | Antigens | Code | Dilution | Supplier |
|---|---|---|---|---|
| Polyclonal rabbit anti-α-transducin | Gαt2 of bovine origin | sc-390 | 1:50 | Santa Cruz |
| Polyclonal rabbit anti-α-gustducin | Gαgust of rat origin | sc-395 | 1:200 | Santa Cruz |
| Polyclonal rabbit anti-somatostatin | Synthetic somatostatin15–28 conjugated to keyhole limpet hemocyanin | 566 | 1:1000 | INCSTAR |
| Monoclonal mouse anti-gastrin/cholecystokinin | Human gastrin/CCK C-terminus | GAS/CCK9303 | 1:1000 | CURE Digestive Diseases Research Center, UCLA |
| Polyclonal goat anti-ghrelin | Human ghrelin | sc-10368 | 1:800 | Santa Cruz |
| Polyclonal rabbit anti-obestatin | Human obestatin | — | 1:1500 | Prof. Rindi G.** |
| Polyclonal rabbit anti-5-hydroxytryptamine | Rat hypothalamus, raphe and spinal cord | 20080 | 1:2000 | INCSTAR |
| Polyclonal rabbit anti-glucagon-like peptide-1 | Synthetic peptide: HDEFERHAEGTFTSDVSSY, corresponding to amino acids 92–110 of Human GLP 1. | Ab22625 | 1:1000 | Abcam |
| Polyclonal rabbit anti-calcitonin gene-related peptide | Synthetic rat CGRP | IHC 6006 | 1:1000 | Peninsula/Bachem |
| Monoclonal rat anti-substance P | Substance-P-BSA conjugate | 10-S15 | 1:300 | Fitzgerald |
| Secondary antisera | ||||
| FITC-conjugated goat anti-rabbit IgG | 1:600 | Calbiochem | ||
| TRITC-conjugated donkey anti-goat IgG | 1:400 | Jackson | ||
| Alexa 594-conjugated goat anti-mouse IgG | 1:400 | Mol. Probes | ||
| Alexa 594-conjugated donkey anti-rat IgG | 1:500 | Mol. Probes | ||
| Biotin-conjugated goat anti-rabbit IgG | 1:400 | Vector | ||
| Texas Red®-conjugated streptavidin | 1:2000 | Vector | ||
**
Kindly provided by Prof. Guido Rindi, Institute of Pathology, Catholic University of the Sacred Heart, Rome, Italy.