Table 2.
Peptide | Code | Concentration | Supplier |
---|---|---|---|
α-transducin | sc-390 P | 20 μg peptide in 1 ml PBS | Santa Cruz Biotechnology, Santa Cruz, USA |
α-gustducin | sc-395 P | 20 μg peptide in 1 ml PBS | Santa Cruz Biotechnology, Santa Cruz, USA |
Ghrelin* | sc-10368 P | 20 μg peptide in 1 ml PBS | Santa Cruz Biotechnology, Santa Cruz, USA |
Glucagon-likepeptide-1 | ab50245 | 10−5 M | Abcam, Cambridge, UK |
Ghrelin peptide was used for both anti-ghrelin and anti-obestatin antibody specificity, since obestatin belongs to the ghrelin peptide family.