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. Author manuscript; available in PMC: 2014 Oct 8.
Published in final edited form as: J Proteomics. 2013 Jun 24;91:10.1016/j.jprot.2013.06.021. doi: 10.1016/j.jprot.2013.06.021

Figure 2. Workflow of the multi-scale strategy.

Figure 2

The top panel shows reversed phase HPLC fractionation of P. interruptus sinus gland tissue extract showing the presence of various sizes of novel neuropeptides. The bottom panel outlines each specific strategy for discovery and identification of small, mid-size and large peptides. It should be noted that the retention behavior of peptides in the reversed phase HPLC is not only based on molecular size, but also the hydrophobicity and other sequence-dependent effects. Here, we show three different color regions in the LC profile for the purpose of illustration.