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. Author manuscript; available in PMC: 2014 Oct 8.
Published in final edited form as: J Proteomics. 2013 Jun 24;91:10.1016/j.jprot.2013.06.021. doi: 10.1016/j.jprot.2013.06.021

Figure 4. Strategy for de novo sequencing of a mid-sized CPRP neuropeptide.

Figure 4

Zoom-in MS spectra of (A) intact Pai−CPRP, (B) dimethylated Pai−CPRP, and (C) proteolytic peptides of Pai−CPRP after Lys-C digestion. The 28 Da of mass increase of peptides arises from N,N-dimethylation.