Table 2.
Tryptic peptides of CHH identified by bottom-up de novo sequencing method.
| Exptl MW | Calcd MW | Sequence a | Tryptic peptide | MS/MS spectrum b |
|---|---|---|---|---|
| 1543.50 | 1543.71 | AVFDQSCKGVYDR | CHH[1-13] | Fig. S7 |
| 2075.66 | 2076.01 | AVFDQSCKGVYDRSLFGK | CHH[1-18] | – |
| 934.36 | 934.52 | SLFGKLDR | CHH[14-21] | Fig. 7C |
| 2420.79 | 2421.15 | SLFGKLDRVCDDCYNLYRK | CHH[14-32] | – |
| 1760.49 | 1760.76 | LDRVCDDCYNLYR | CHH[19-31] | Fig. S7 |
| 1888.53 | 1888.85 | LDRVCDDCYNLYRK | CHH[19-32] | – |
| 1376.35 | 1376.55 | VCDDCYNLYR | CHH[22-31] | Fig. S7 |
| 1504.39 | 1504.64 | VCDDCYNLYRK | CHH[22-32] | Fig. S7 |
| 2358.16 | 2358.15 | KHYVSTGCRKNCYGNLVFR | CHH[32-50] | – |
| 2230.06 | 2230.05 | HYVSTGCRKNCYGNLVFR | CHH[33-50] | Fig. 7B |
| 1269.64 | 1269.63 | KNCYGNLVFR | CHH[41-50] | Fig. 7D |
| 1141.35 | 1141.53 | NCYGNLVFR | CHH[42-50] | Fig. S7 |
a
Fixed carbamidomethyl modification is on cysteine. The residues different from the homologous sequence are highlighted with underline.
b
Data is selectively shown for covering the sequence CHH[1-50]. CHH[51-71] was determined by top-down de novo sequencing.