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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: DNA Repair (Amst). 2013 Aug 26;12(11):10.1016/j.dnarep.2013.07.008. doi: 10.1016/j.dnarep.2013.07.008

Figure 7.

Figure 7

Summary of the relative contributions of alternative TLS mechanisms toward mutagenesis at abasic sites. (A) In WT and rad30, TLS dependent on Pol ζ and the structural function of Rev1 is necessary for the majority of A insertion events, while C insertion events require the presence of Pol ζ and fully functional Rev1. The relative frequencies of the two outcomes are roughly equal. (B) In rev3 and rev1, C insertion events are not observed. The observed A insertion events are independent of Pol ζ and Rev1, and might represent low-frequency TLS bypass by a replicative polymerase (presumably Pol δ) acting alone. (C) In rev1-AA, only A insertion events, which require Pol ζ and Rev1 structural function, are observed. The complete lack of C insertion events in rev1-AA implies that Rev1’s deoxycytidyltransferase function is responsible for such events in WT. (D) In pol32, both Pols δ and ζ are structurally incomplete. This presumably results in low-frequency, but observable TLS events that insert either A or C. (E) In pol32 rev1-AA, only low-frequency A insertion events are observed, involving incomplete Pol ζ and Rev1 structural function.