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. Author manuscript; available in PMC: 2014 Nov 15.
Published in final edited form as: J Neurol Sci. 2013 Aug 2;334(0):10.1016/j.jns.2013.07.008. doi: 10.1016/j.jns.2013.07.008

Table 2.

Primers used for real-time PCR Assays

Gene Primers (forward/reverse, 5′-3′) Detection
NK1R-F TCTTCTTCCTCCTGCCCTACATC
GCCCAGACGGAACCTGTCAT		 56-FAM-CCAGATCTCTACCTGAAGAAGTTTATCCAGCA-3BHQ1
NK1R-T TCTTCTTCCTCCTGCCCTACATC
TGG AGAGCTCATGGGGTTGGGATCCT		 5′ FAM-CCAGATCTCTACCTGAAGAAGTTTATCCAGCA-3′ BHQ1
TAC-1 (SP) CGGACCAGTAATTCAGATCATCA
GAGGAACCAGAGAAACTCAGC		 56-FAM-CATGTTGGA/ZEN/TTTTGCGACGGACAGT/3IABkFQ
CCR5 CAAAAAGAAGGTCTTCATTACACC
CCTGTGCCTCTTCTTCTCATTTCG		 SYBR
CD4 GGAAAGAAAGTGGTGCTGGGCAAA
TCCTTGGTCCCAAAGGCTTCTTCT		 SYBR
CXCR4 TGGCCTTATCCTGCCTGGTATTGT
AGGAGTCGATGCTGATCCCAATGT		 SYBR
Fractalkine GAGCCGACTCCTTCTTCC
CCTCCATCCTGAGCCTTT		 SYBR
IL-6 AGTGAGGAACAAGCCAGAGC
GTCAGGGGTGGTTATTGCAT		 SYBR
CCL2 (MCP-1) CTCGCTCAGCCAGATGCAATCAAT
TGGAATCCTGAACCCACTTCTGCT		 SYBR
CCL20 (MIP3A) AGTTTGCTCCTGGCTGCTTTGATG
CTGCCGTGTGAAGCCCACAATAAA		 SYBR
IL-10 GTGATGCCCCAAGCTGAGA
CACGGCCTTGCTCTTGTTTT		 56-FAM-CCAAGACCCAGACATCAAGGCGCA-3BHQ1
CD163 AAAAGCGAAGACAGAGACAGC
ATCATCTGCATTCAGGCAAG		 SYBR
HIV-gag [37] CATGTTTTCAGCATTATCAGAAGGA
TGCTTGATGTCCCCCCACT		 56-FAM-CACCCCACAAGATTTAAACACCATGCTAA-3BHQ1
GAPDH GTGGTCTCCTCTGACTTCAACA
TGCTGTAGCCAAATTCGTTG		 56-FAM-CTGGCATTGCCCTCAACGACC-3BHQ1