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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Aug 3;1832(12):10.1016/j.bbadis.2013.07.020. doi: 10.1016/j.bbadis.2013.07.020

Figure 2. Nipbl deficiency does not increase DNA damage sensitivity in MEFs.

Figure 2

(A) Survival of wild-type and Nipbl heterozygous cells after exposure to the DNA damaging agents aphidicolin, hydroxyurea, mitomycin C and gamma irradiation at the indicated doses (n=2 clones per genotype). (B) Left: 53BP1 staining of G2 irradiated (8Gy) cells, identified as pH3(S10) positive, was categorized in three different classes: homogenous nuclear staining (class I), few foci with diffuse staining (class II) and many strong foci without diffuse staining (class III). Right: Quantification of these phenotypes before, immediately after and 3 hours after irradiation of wild-type and Nipbl heterozygous MEFs. (n ≥ 50 G2-cells per condition and per clone from two independent clones per genotype).