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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Aug 3;1832(12):10.1016/j.bbadis.2013.07.020. doi: 10.1016/j.bbadis.2013.07.020

Figure 3. Nipbl deficiency does not affect bulk cohesin loading.

Figure 3

Asynchronous cultures of two clones per genotype were subjected to chromatin fractionation followed by immunoblotting with the indicated antibodies. Mek2 cytoplasmatic kinase and histone H3 are used as control for the fractionation procedure. WCE whole-cell extract; Cyt, cytoplasm; Chr, chromatin-enriched fraction. The soluble nucleoplasmic fraction was not loaded in this gel. In the immunoblot of Wapl, the lower band indicated by the asterisk correspond to SA2, since the membrane was reused.