S6K and TNF-α level in p50-KO mice. (A) S6K protein level in tissues. S6K protein was examined in the white adipose tissue (WAT), liver, and muscle of WT and p50-KO mice. (B) S6K mRNA level was determined by qRT-PCR in the liver, muscle and WAT. (C) TNF-α in p50-KO mice. TNF-α protein was determined in the serum by ELISA. TNF-α mRNA was determined in the liver, muscle and WAT with qRT-PCR. (D) JNK and IKKβ in liver. Liver tissues of p50-KO mice were examined for JNK, IKK2 and NF-kB p65 and p50. The experiments were conducted 3 times with consistent results and the representative blots are presented. In the bar figure, each data point represents mean ± SEM (n=6). * P<0.05, ** P<0.001.