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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Jun 28;1832(12):10.1016/j.bbadis.2013.06.013. doi: 10.1016/j.bbadis.2013.06.013

Fig. 3.

Fig. 3

S6K-JNK interaction in cells. (A) Association of S6K and JNK in HEK 293 cells. The cells were transfected with HA-S6K and treated with TNF-α. Immunoprecipitation was conducted with HA antibody or JNK antibody. The product was blotted for endogenous JNK or HA-S6K in an immunoblot. (B) Inhibition of S424 phosphorylation by JNK inhibitor. HEK 293 cells were pretreated with JNK inhibitor SP600125 (SP, 25μM), mTOR inhibitor Rapamycin (Rap, 200 nM), p38 inhibitor SB203580 (SB, 5 μM) or ERK inhibitor PD98059 (PD) and then examined for S6K phosphorylation after TNF-α treatment. Upper: pS6K (S424) and pS6K (T389) were examined in a Western blot. The representative blots are presented. Lower: quantification of S424 phosphorylation in three plots. The relative signal intensity at each lane was normalized with the S6K1 protein. Each data points represents mean ± SEM (n=3). The experiments were repeated three times with consistent results and representative blots are presented in this figure. * P<0.05 over control.