Fig. 4.

TNF-α induce S6K degradation in KO cell lines. (A) S6K in wild type cells. Wild type cell lines were treated with TNF-α (20 ng/ml) for indicated time. S6K and its phosphorylation were determined in the whole cell lysates in an immunoblot for S6K, pS6K(S424), pS6K(T389), and pc-JUN. (B) S6K degradation in p50-KO MEF cells. (C) S6K degradation in IKK2 null cells. (D) S6K degradation in p65 null cells. (E) Inhibition of S6K degradation by MKK7 knockdown in p65-KO cells. MKK7 was knocked down using siRNA in p65-KO cells. The experiments were repeated three times with consistent results and representative blots are presented in this figure.