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. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: Anal Biochem. 2013 Sep 14;443(2):10.1016/j.ab.2013.09.009. doi: 10.1016/j.ab.2013.09.009

Figure 6.

Figure 6

Specific and non-specific interaction of vincristine, vinblastine, BODIPY FL Vinblastine, BODIPY FL propionic acid, BODIPY FL hydrazide or BODIPY FL EDA with 5 nM GST-hPXR-LBD and 5 nM Tb-anti-GST after 30 minutes of incubation. (A) Competition of TO901317, vincristine, or vinblastine with 250 nM BODIPY FL Vinblastine in the presence of 5 nM GST-hPXR-LBD and 5 nM Tb-anti-GST. (B) Structures of BODIPY FL propionic acid, BODIPY FL hydrazide, and BODIPY FL EDA. (C) Interaction of 250 nM BODIPY FL Vinblastine, BODIPY FL propionic acid, BODIPY FL hydrazide, or BODIPY FL EDA with 5 nM Tb-anti-GST with or without 5 nM GST-hPXR-LBD in the presence of DMSO or 50 μM TO901317. The inset shows an enlargement of the BODIPY FL Vinblastine bar graph.