Fig. 7. Effect of granulocyte macrophage colony-stimulating factor (GM-CSF) on melan-A melanocyte proliferation. (A) Melan-A melanocytes (1×104 cells/well) were cultured in 24 well plates. After incubation with 400, 200, 100, 50, 25, or 12.5 pg/mL GM-CSF for 24, 48, and 72 h, cell proliferation was measured as described in the Materials and Methods section section. (A) Control, (B) GM-CSF (25 pg/mL), and (C) cell proliferation assay. Cells were observed under a phase-contrast microscope (Olympus CKX41, Olympus Optical, Tokyo, Japan) and images were photographed using a DMCe camera (INS Industry, Seoul, Korea) and the included DMC advanced software (×100). Data are shown as average±SEM. PBS, phosphate buffered saline. *p<0.05, **p<0.01, compared with the control.
