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. 2000 Jan 1;105(1):79–92. doi: 10.1172/JCI8066

Figure 1.

Figure 1

S. typhimurium and TNF-α induce IL-8 expression by an NF-κB–mediated mechanism. (a) EMSAs with an IL-8 NF-κB motif probe. Nuclear extracts were derived from model intestinal epithelia maintained under control conditions or coincubated with wild-type S. typhimurium as described in Methods for the indicated times. Addition of unlabeled competitor probe or antisera when indicated. Inset: nuclear extracts were Western blotted with anti-p65 as described in Methods. (b) Transient transfection assay with IL-8 reporter constructs. Transfected HT-29 cl19A cells were treated as indicated. Data are reported as fold induction of CAT activity over basal. (c) IL-8 ELISA from model epithelia. Cells were incubated with basolateral TNF-α (10 ng/mL) or apical wild-type S. typhimurium, either with or without 1 hour pretreatment with 50 μM MG-132. Basolateral IL-8 secretion was measured 5 hours later.