Figure 1.

Expression of p110α-RBD Induces Tumor Regression in Early-Stage Tumors

(A) Schematic representation of experimental conditions.

(B) Representative images of 4-week-old and 16-week-old mouse lungs treated and untreated with tamoxifen (TX). Graph showing tumor number on the pleural surface of lungs. Four-week-old group: Pik3ca^WT/flox n = 7 mice, Pik3ca^RBD/flox n = 11; 16-week-old group: Pik3ca^WT/flox n = 9, Pik3ca^RBD/flox n = 12, Pik3ca^WT/- n = 10, Pik3ca^RBD/- n = 11 mice.

(C) Representative H&E-stained lung sections from 4-week-old and 16-week-old mice. Representation of tumor burden (tumor area as a percentage of total lung area) in 4-week-old mice and in 16-week-old mice 12 weeks after tamoxifen treatment.

(D) Representation of average tumor size from the same group of mice mentioned above.

(E) Quantification of TUNEL positive tumors in 16 weeks old Pik3ca^RBD/− and Pik3ca^WT/− mice.

(F) Analysis of the proliferative state of Pik3ca^WT/− and Pik3ca^RBD/− tumors using phospho-histone H3 staining.

Error bars indicate mean ± SEM (significance using Student’s t test: ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001). See also Figure S1.