Figure 2.

Expression of p110α-RBD in Established Tumors Reduces Tumor Burden

(A) Schematic representation of mouse treatment and micro-CT scanning intervals.

(B) Ten-week-old mice were left untreated or were treated with tamoxifen for 2 weeks and the effect on tumor volume was assessed by micro-CT analysis over a 6-week period. Tumor volumes are plotted relative to the initial volume at the start of treatment. Black lines represent tumors from Pik3ca^WT/− mice and red lines represent tumors from Pik3ca^RBD/− mice. Green lines represent tumors from tamoxifen-treated Pik3ca^WT/WT mice that express Cre recombinase following treatment but do not have loxP sites flanking the Pik3ca gene. Pik3ca^WT/flox n = 4 mice, Pik3ca^RBD/flox n = 4, Pik3ca^WT/- n = 6, Pik3ca^RBD/- n = 4, Pik3ca^WT/WT n = 3 mice.

(C) Quantification of TUNEL-positive tumors following tamoxifen treatment for 11 days. Pik3ca^WT/− n = 5 mice, Pik3ca^RBD/− n = 7 mice

(D) Quantification of tumor multiplicity on the pleural surface of lungs. Representative images of lungs from Pik3ca^WT/flox, Pik3ca^RBD/flox, Pik3ca^WT/−, and Pik3ca^RBD/− mice at 16 weeks of age are shown.

(E) Quantification of tumor burden (tumor area as a percentage of total lung area) in mice 6 weeks after commencement of tamoxifen treatment. Representative images of histological sections stained with H&E in Pik3ca^WT/flox, Pik3ca^RBD/flox, Pik3ca^WT/−, and Pik3ca^RBD/− mice at 16 weeks of age are shown.

Error bars indicate mean ± SEM (significance using Student’s t test: ^∗p < 0.05 ^∗∗p < 0.01). See also Figure S2.