Figure 4.

Deficient Tumor Growth in p110α-RBD Mutant Mice Is a Cell-Autonomous Process

(A) 3D models showing the growth of transplanted Pik3ca^WT/− and Pik3ca^RBD/− tumor cells in the lung based on micro-CT images. Due to the tumor occupying the complete lobe at 12 weeks after treatment in Pik3ca^WT/− inoculated lungs, modeling was not possible.

Representative images of Pik3ca^WT/− and Pik3ca^RBD/− tumor cell inoculated lungs 14 weeks after tamoxifen treatment are shown in the most right panels.

(B) Mice were scanned 6 and 12 weeks after tamoxifen treatment. The graph represents the normalized air volume in the lungs at the moment of the scanning. Black lines represent air content in NuNu mice bearing Pik3ca^WT/−-derived tumors and red lines represent air content in mice bearing Pik3ca^RBD/−-derived tumors.

Representative tomographs from micro-CT analysis of Pik3ca^WT/−-derived and Pik3ca^RBD/−-derived tumors are shown at the right.

(C) Representation of lifespan of NuNu mice bearing either Pik3ca^WT/−-derived or Pik3ca^RBD/−-derived tumors. NuNu mice containing Pik3ca^RBD/−-derived tumors were sacrificed when the last mouse from the control group had to be culled because of breathing problems.

(D) Representative images of histological sections stained with H&E in mice inoculated with Pik3ca^WT/−-derived and Pik3ca^RBD/−-derived tumor cells 14 weeks after tamoxifen treatment.