Fig. 2.

Comparison of the influence of 7-ketocholesterol (10 ug/ml) and 25-hydroxycholesterol (10 ug/ml) on human aortic endothelial cells (HAEC) apoptosis (24 h) assessed by the flow cytometry. a Ax−Pi− Annexin-V-negative, propidium iodide-negative cells; Ax+Pi− Ax+Pi+ Annexin-V-positive, propidium iodide-negative cells plus Annexin-V-positive, propidium iodide-positive cells; n = 5, from 5 independent experiments, mean ± SEM; *p < 0.05. b Representative dot blots of unstimulated (unst), 25-hydroxycholesterol-induced (25-OHCH) and 7-ketocholesterol-induced (7-kCH) Caco-2 cells; x axis Annexin V-FITC, y axis propidium iodide, flow cytometry