Fig. 4.

Comparison of 7-ketocholesterol (10 ug/ml) and 25-hydroxycholesterol (10 ug/ml) influence on intestinal epithelial Caco-2 cell apoptosis (24 h) assessed by flow cytometry. a Ax– Pi− Annexin-V-negative propidium iodide-negative cells; Ax+Pi− Ax+Pi+ Annexin-V-positive, propidium iodide-negative cells plus Annexin-V-positive, propidium iodide-positive cells; n = 5, from 5 independent experiments, mean ± SEM; *p < 0.05). b Representative dot blots of unstimulated (unst), 25-hydroxycholesterol-induced (25-OHCH) and 7-ketocholesterol-induced (7-kCH) Caco-2 cells; x axis Annexin V-FITC, y axis Propidium iodide, flow cytometry